Recent microRNA Publications from our Lab
1: Trifunovic A, Dombkowski A, Cukovic D, Mahajan P. The Potential of microRNAs as Non-invasive Biomarkers in Acute Pediatric Asthma. J Allergy Clin Immunol. 2020 PMID: 32018032
2: Dombkowski AA, Cukovic D, Bagla S, Jones M, Caruso JA, Chugani HT, Chugani DC. TLR7 activation in epilepsy of tuberous sclerosis complex. Inflamm Res. 2019 PMID: 31511910
3: Bagla S, Dombkowski AA. Neuroinflammatory Nexus of Pediatric Epilepsy. J Pediatr Epilepsy. 2018 PMID: 31709125
4: Dombkowski AA, Batista CE, Cukovic D, Carruthers NJ, Ranganathan R, Shukla U, Stemmer PM, Chugani HT, Chugani DC. Cortical Tubers: Windows into Dysregulation of Epilepsy Risk and Synaptic Signaling Genes by MicroRNAs. Cereb Cortex. 2016, PMID: 25452577
5: Bagla S, Cukovic D, Asano E, Sood S, Luat A, Chugani HT, Chugani DC, Dombkowski AA. A distinct microRNA expression profile is associated with α[(11)C]-methyl-L-tryptophan (AMT) PET uptake in epileptogenic cortical tubers resected from patients with tuberous sclerosis complex. Neurobiol Dis. 2018, PMID: 28993242
6: Farran B, Dyson G, Craig D, Dombkowski A, Beebe-Dimmer JL, Powell IJ, Podgorski I, Heilbrun L, Bolton S, Bock CH. A study of circulating microRNAs identifies a new potential biomarker panel to distinguish aggressive prostate cancer. Carcinogenesis. 2018 PMID: 29471417;
7: Dyson G, Farran B, Bolton S, Craig DB, Dombkowski A, Beebe-Dimmer JL, Powell IJ, Podgorski I, Heilbrun LK, Bock CH. The extrema of circulating miR-17 are identified as biomarkers for aggressive prostate cancer. Am J Cancer Res. 2018, PMID: 30416858
microRNAs in Human Diseases
MicroRNAs are short, non-coding oligonucleotides that regulate many genes by suppressing protein translation or through transcript degradation. Nearly every biological process thus far investigated is regulated, in part, by microRNAs. Consequently, aberrant microRNA activity is associated with many diseases and pathological events. Mature microRNAs are approximately 22 nucleotides in length and anneal to complementary sites in the 3’ untranslated region (UTR) of target transcripts as part of an RNA-induced silencing complex (RISC). There are currently more than 2000 known microRNAs in the human genome, and each microRNA may regulate dozens to hundreds of target transcripts. The 3’ UTR in a single messenger RNA may contain binding sites for numerous microRNAs, and a transcript can be concurrently repressed by multiple microRNA species. Therefore, microRNAs are part of a highly complex gene regulatory network. To improve our detection and treatment of diseases it is important to untangle this network and understand how microRNAs are involved in disease processes.
microRNAs as Biomarkers in Pediatric Asthma
Our study demonstrated the potential of using microRNAs obtained from non-invasive nasal swabs as biomarkers of asthma severity. We found that microRNA miR-582-5p was significantly increased in the nasal epithelium of pediatric patients having severe asthma.
Trifunovic A, Dombkowski A, Cukovic D, Mahajan P. The Potential of microRNAs as Non-invasive Biomarkers in Acute Pediatric Asthma. J Allergy Clin Immunol. 2020 PMID: 32018032